日本凋毛藻碳酸酐酶基因及其编码的蛋白特征

碳酸酐酶CA(EC 4.2.1.1)是含锌原子的金属蛋白酶,该酶的基本功能是催化CO₂与HCO₃-间的相互转换[1].该反应在许多生物过程中都发挥着重要的作用,例如离子交换、呼吸作用、pH稳定性、捕获CO₂和光合作用等[2~5].CA广泛分布于各种生物中,已知的CA酶根据氨基酸序列被分为-αCA,-βCA和γ-CA三种类型[5~7].这3种类型的CA酶序列间的相似性很低,被认为是由不同的途径进化而来的[7].α-型的CA酶在动物、植物、绿藻、细菌和蓝细菌中都有发现[7],在藻类中报道的α-型CA还很少.迄今为止只在衣藻中发现2种异构体,在盐藻中发现3种[8,9],在红藻中还未见报道.     

东海秋季典型站位沉降颗粒物通量

2002年9月在东海的长江口、中陆架区和浙江近岸上升流区三个站位放置沉积物捕获器采集沉降颗粒物。在对颗粒有机碳(POC)、颗粒有机氮(PON)和总颗粒碳(PC)元素分析基础上,采用颗粒物通量模型对沉降通量进行了研究。镜检发现细小无机颗粒物和大颗粒聚合体是三个站位沉降颗粒物的主要形式。大颗粒聚合体有住囊类、粪球聚合体、硅藻聚合体和混杂聚合体四种类型。研究结果显示,东海中陆架区和浙江近岸上升流区沉降颗粒物中POC、PON和PC的百分含量均呈现随水深增加明显降低的趋势,但在长江口,这些成分的含量低且上下均匀。长江口观测到的是大风后的一个实例,存在强烈的再悬浮,各水层颗粒物沉降通量平均(±SE)高达(319.02±65.33)g/(m2.d),尽管如此,沉降颗粒物有机态C/N值却很高(18.0±0.9),明显受陆源颗粒物的影响。POC净沉降通量在浙江近岸上升流区为961mg/(m2.d)(水深55m),在东海中陆架区为123mg/(m2.d)(水深88m),可见浙江近岸上升流区是POC向海底转移的重要区域之一,其垂直转移能力明显高于东海中陆架区。在上升流区域和中陆架区,POC的输出比率大约分别为48%—77%和15%—21%。浙江近岸上升流区和东海中陆架区底层颗粒物再悬浮比率分别为66.50%和88.52%。研究显示,浙江近岸上升流区的水体底层颗粒物受底部平流的影响比东海中陆架区相对较强。     

乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达

采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。     

荧光法在赤潮图像分析中的应用

赤潮藻样品中往往包含着各种复杂的非藻沉淀物,为解决这些微藻的探测和分析的问题,可以利用微藻类在一定波长的光照射下会产生荧光的特性,对裸甲藻等多种赤潮藻类进行荧光实验处理,并在此基础上对藻类的荧光图像和灰度图象进行图像分割,轮廓和纹理分析及图象识别等后继图象处理。实验证明,荧光法可以有效的去除背景噪声,保留藻类信息,使得后继的图象处理工作更加容易和有效,从而解决了探测和分析包含复杂背景的沉淀物样品中微藻的问题。     

Three-dimensional numerical simulation of oblique wave action on vertical walls

1 Introduction A vertical wall is one of the typical maritime sheltering structures in the coastal region, which is mainly subjected to wave forces. It has been a com- mon engineering assumption adopted for design pur- poses that normally incident wave fo…     

Impacts of TRMM SRR assimilation on the numerical prediction of tropical cyclone

1 IntroductionObservation of the tropical rainfall is crucial forthe research on tropical weather and climate. Nu-merous studies have shown that the ingestion of rain-fall data into a numerical model can have considera-ble impacts on simulation results(Kr…     

Sedimentary facies and paleoenvironmental interpretation of a Holocene marsh in the Gironde Estuary in France

1Introduction Since the last glacial maximum, the Holocenehas been marked by a rapid rise in sea level. After6 000 a BP, the present-day level was reached andthe rate of sea-level rise (SLR) decreased rapidly(Morzadec -Kerfourn, 1974; Kidson, 1986 ).These…     

海洋渔业资源可持续利用的一种模式——海洋牧场

介绍了海洋牧场的发展史和国外海洋牧场建设状况,并结合我国的海洋渔业形势,分析了海洋牧场与“海洋农牧化”之间的关系,以及在我国建设海洋牧场的重要性和迫切性。就海洋牧场的建设内容以及可行性进行了分析与论述,希望能对我国海洋渔业资源的扩充提供一个新的思路!     

Study on copper complexing ligand concentrations in several China’s coastal waters

Copper complexing ligand concentrations in the Daya Bay, Qingdao coast, Jiaozhou Bay, South China Sea and Huanghe Estuary waters were determined by the anodic stripping voltammetry technique. The distribution regularity and the relationship with other parameters were discussed. The results were as follows: Copper complexing ligand concentrations of the South China Sea were a little higher than those of other sea areas, and they were apparently higher than those of the ocean. Compared with the subsurface layer (SSL) in the sea surface microlayer copper complexing ligand concentrations showed an enrichment phenomenon, of which the mechanism is similar to dissolved organic matter. The metal complexing ligand concentration profiles of the South China Sea showed that the value in the sea surface was the highest, then it decreased with depth accruing, and a higher value appeared at the bottom. Copper complexing ligand concentrations were higher than those of cadmium and lead. Ligands in each sea area exhibited a complicated property. In short, the distribution regularity of copper complexing ligand concentrations in China' s coastal waters was consistent with that of other regions in the world. Meanwhile, the positive relationship between the copper complexing ligand concentrations and biological oxygen demand, chemical oxygen demand, dissolved organic carbon, and viscosity were found clearly.     

Long baroclinic Rossby waves with periods of about 500d near 20°N in the northwest Pacific Ocean

On the basis of maps of sea level anomalies data set from October 1992 to January 2004, pronounced low frequency variations with periods of about 500 d are detected in the area near 20°N from 160°W to 130°E. A linear two-layer model is employed to explain the mechanism. It is found that the first-mode long baroclinic Rossby waves at 20°N in the northwest Pacific propagate westward in the form of free waves at a speed of about 10.3 cm/s. This confirms that the observed low frequency variabilities appear as baroclinic Rossby waves. It further shows that these low frequency variabilities around 20°N in the northwest Pacific can potentially be predicted with a lead up to 900 d.